Widal Test (Slide Agglutination)

Also referred as

Typhoid Test (Slide Agglutination)

For men & women

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₹160

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Know more about this test

Widal Test (Slide Agglutination) test helps to detect typhoid fever and paratyphoid fever – collectively known as enteric fever, caused by the consumption of food or water contaminated by Salmonella bacteria. The symptoms include high fever, malaise, headache, constipation or diarrhoea, rose-colored spots on the chest, and enlarged spleen & liver.

This test helps to detect antibodies in a blood sample against two antigens (O & H) of bacteria. It should only be performed after a week or two for accurate results.

Please consult your health care practitioner to get an accurate diagnosis based on the symptoms, medical history and test results.

Preparation for this test

Sample Collection

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Tata 1mg Labs

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Verified reports

Who will collect your samples?

Tata 1mg certified phlebotomists

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Tata 1mg Labs (Tata 1mg Technologies Private Limited)

NABL certified, ISO certified

About this lab

TATA 1mg labs is a state-of-art facility offering the highest quality diagnostic services at the convenience of your doorstep. We pride ourselves on three things 1) Assured Quality 2) Best Prices 3) Excellent Turn Around Time. We believe in providing the highest level of transparency to our customers. Testimony to the quality is the ISO certification, a gold standard in the quality of diagnostics. Our entire team is dedicated to providing the best customer experience and continuously strives to come up with solutions to remain in line with the needs of our customers.

What does Widal Test (Slide Agglutination) measure?

The Widal test measures the titres of antibody against the bacteria which cause Enteric fever.

Typhoid and paratyphoid fever are generally acquired when you consume food or water, contaminated by feces of an acutely infected or convalescent person (recovering from disease) or a chronic, asymptomatic carrier. The incubation period (the time interval between exposure to an infection and the appearance of the first symptoms) of Enteric fever is 6-30 days. Paratyphoid fever is similar but often less severe than typhoid fever.

Widal test is an agglutination test to detect antibodies (agglutinins) in a blood sample against two antigens (O & H) of bacteria, Salmonella enterica. Agglutination refers to the visible clumping of particles when a particulate antigen combines with its antibody in the presence of optimum conditions for antigen-antibody reaction. When this test is performed on a slide, it is called Slide agglutination and when it is carried out in a test tube, it is called Tube agglutination. Widal test by Tube agglutination is recommended over Slide agglutination method. The antigens used in the test are “H” and “O” antigens of Salmonella Typhi and “H” antigen of S. Paratyphi.

Widal test should only be performed after the first week. The reason being the antibody against “O” and “H” antigens of Salmonella start appearing in serum at the end of the first week of fever. It is preferable to test two blood samples at an interval of 7 to 10 days to demonstrate rising antibody titres.

Test Information is reviewed by: Dr. Swati Gupta (Anand), MBBS, MD Lab Medicine and written by Dr. Lipika Khurana, BDS, PGDHHM . The test details are for information purpose only. Consult a doctor before taking any test. Last updated on: 15th Apr 2026

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Widal Test (Slide Agglutination) test price for other cities

Price in	Ahmedabad	Rs. 160
Price in	Allahabad	Rs. 160
Price in	Bangalore	Rs. 160
Price in	Bhopal	Rs. 160
Price in	Chandigarh	Rs. 160

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FAQs related to Widal Test (Slide Agglutination)

Is there any preparation required before the test?

Inform the doctor about the medications you may be taking. No other specific preparations are usually required before this test.

What are the different methods for performing Widal test?

Widal test can be performed by the following two different methods: 1. Slide agglutination Widal test Qualitative Slide Test Quantitative Slide Test 2. Tube agglutination Widal test Tube agglutination is the recommended method as it has more accuracy. However, Slide agglutination is preferred by some as it gives the rapid result. Tube agglutination method requires approximately 18 hours for test completion.

Is there any risk associated with this test?

There is no risk associated with the test. However, since this test involves a needle prick to withdraw the blood sample, in very rare cases, a patient may experience increased bleeding, hematoma formation (blood collection under the skin), bruising or infection at the site of needle prick.

What are the limitations of Widal test?

The limitations of Widal test are: Previous typhoid vaccination may result in increased antibody titres in the non-infected persons False positive Widal test can occur in patients with non-enteric Salmonella infection, for example Typhus, Immunological disorders, chronic liver disease, Malaria and cryptococcal meningitis Prior use of antibiotics can affect antibody response giving a low titre in the Widal test even in the presence of bacteriologically confirmed typhoid fever The Widal agglutination titre varies with the geographic location based on the prevalence of Salmonella infection in that area Past infection with serotype Typhi or another nontyphoidal Salmonella serotype also results in false positive Widal test

What are the other tests to diagnose Enteric fever?

The other tests which are used to diagnose Enteric fever include Blood culture test, Widal test, Stool culture test, and Urine culture test. Among these, blood culture is the most commonly recommended method for diagnosing Enteric fever and is performed during the first week of fever. If in case other test results come inconclusive then bone marrow samples can be used to diagnose any infections.

How can you prevent typhoid fever?

Avoiding contaminated foods and drinks can help to prevent typhoid fever. Along with that, proper vaccination against typhoid fever can also keep a check on developing any possible case of infection.

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