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HLA B-27, Flowcytometry

HLA B-27, Flowcytometry

For men & women
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HLA B-27, Flow Cytometry test is performed to measure the level of HLA B27 antigen in blood. Presence of HLA B-27 may indicate a possibility of autoimmune disease, where the guarding cells (WBC) attacks own cells.
This test is done if one experiences signs and symptoms of arthritis such as chronic joint pain, inflammation in the lower back and hips. It can also help detect inflammatory bowel disease and other chronic conditions.
People with a positive HLA B-27 have an increased chance of developing autoimmune diseases like rheumatoid arthritis, juvenile idiopathic arthritis, and ankylosing spondylitis.


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What does HLA B-27, Flowcytometry measure?

HLA B-27 test determines the presence or absence of human leukocyte antigen which is present on the surface of white blood cells. The HLA B-27 also refers to the gene which codes for the HLA B-27 protein. 


HLA B-27 are proteins which help the immune system to differentiate the body’s own cells from other foreign cells. Every person has an inherited combination of HLA genes which codes for many antigens which are present on the surfaces of their cells. This HLA combination is distinctive for each person and is based on the presence or absence of each antigen.


People with HLA B-27 have increased chances of developing autoimmune diseases like ankylosing spondylitis, rheumatoid arthritis, reactive arthritis, and isolated acute anterior uveitis. HLA B-27 is also said to be linked with inflammatory bowel disease and other chronic conditions.


FAQs related to HLA B-27, Flowcytometry

Inform the doctor about the medications you may be taking. No other specific preparations are usually required before this test.
Ankylosing spondylitis is a form of arthritis that primarily affects the spine, but can affect other joints as well. It comprises of vertebrae (spinal joints) inflammation which can further cause severe, chronic pain and discomfort. If the inflammation is left unchecked, it can lead to new bone formation in the spine which is known as ankylosis. In ankylosis, sections of the spine fuse in a fixed and immobile position. In this, one can develop inflammation, pain and stiffness in other parts of the body such as hips, ribs, shoulders, heels, and small joints of hands and feet. The inflammation can also involve eyes, the condition is known as uveitis or iritis. Whereas, in rare cases, heart, and lungs can be affected.
Reactive arthritis is a painful form of inflammatory arthritis. This includes joint disease caused due to inflammation. It develops due to inflammation caused in response to infection which is caused by certain bacteria which are present in genitals (Chlamydia trachomatis) or the bowel (Campylobacter, Shigella, Salmonella, and Yersinia).
The healthcare provider takes a blood sample from the arm. The site from where the blood is to be withdrawn is cleaned with a swab of rubbing alcohol. This is then followed by inserting a small needle which has a tube attached to it for collecting blood. Once the sufficient blood for analysis is withdrawn, the needle is removed. The site is then covered with a gauze pad.
As such there is no risk but in few cases, bruising, bleeding, and infection at the puncture site can be seen. In very few cases, there can be swelling of the vein after the blood is withdrawn.
Content created by
Written by:
Dr. Lipika Khurana
BDS, PGDHHM
Reviewed by:
Dr. Swati Gupta (Anand)
MBBS, MD Lab Medicine
Want to know moreRead our editorial policy
Last Updated9th Feb 2026

HLA B-27, Flowcytometry test price for other cities

Price inAhmedabadRs. 1820
Price inAllahabadRs. 1820
Price inBangaloreRs. 1820
Price inBhopalRs. 1820
Price inChandigarhRs. 1820

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HLA B-27, Flowcytometry